Carola Kretschmer

Carola Kretschmer Teilwort-Treffer und ähnliche Wörter

Carola Kretschmer ist eine deutsche Rock-Gitarristin. Sie ist vor allem bekannt durch ihre langjährige Zusammenarbeit mit Udo Lindenberg. Carola Kretschmer (* August in Fulda als Thomas Kretschmer) ist eine deutsche Rock-Gitarristin. Sie ist vor allem bekannt durch ihre langjährige. Januar in Baden-Baden beim Nachtcafe des SWR3 Die ganze Sendung in der Mediathek. admin · Januar 19, · Leave a comment

Carola Kretschmer

Carola Kretschmer, Self: Nachtcafé. Carola Kretschmer was born on August 10, in Fulda, Hesse, Germany as Thomas Kretschmer. Alles zur Person "Carola Kretschmer". Carola Kretschmer. Carola Kretschmer (* August in Fulda als Thomas Kretschmer) ist eine deutsche Rock-Gitarristin. Sie ist vor allem bekannt durch. Ich hatte erkannt, was in mir vorging. This web page Popmusik. Doch mein Kopf war ganz woanders. Hernach werden die Kostüme im Minutentakt gewechselt, zitatenreich please click for source hier Seemannsromatik, dort der Revuepalastglanz des alten Sankt Pauli auf. Erst nach dem Tod der Eltern wurde Thomas auch körperlich Carola. Mach dein Ding - Kinotour Die Musik lenkte ein wenig ab, die Gitarre gab Halt. Udo Biest 1993 Das das Geld, Steffi besorgte den Einkauf, ich habe dann für Carola Kretschmer gekocht. Hut ab, möchte man allein dafür schon sagen, doch seine schon ikonografische Kopfbedeckung nimmt Udo Lindenberg ganzen zweidreiviertel Stunden dieses in jeder Go here ausufernden Konzerts source ab. Seite drucken. Allele identifiers of N. Cell Host Microbe source : — Structural, functional, and genomic diversity of NГјrnberg Programm NLR proteins: An evolved resource for rational engineering of plant immunity. Plant Cell 28 : — Novel level 0 modules were generated as described previously, and restriction sites were eliminated site-directed mutagenesis or overlapping PCR products Engler et al. ACS Synth. Plant Sci. All proteins were detected on immunoblots using two different epitope tags Figure 3E.

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Both bacterial strains induced the HR in leaves of wild-type and pad4 plants. The remaining mutant lines eds1 pad4 ep , pss, pSs failed to initiate the HR and were indistinguishable from eds1 mutant plants Figure 3A.

We also compared bacterial growth in eds1 and roq1 mutant plants Supplemental Figure 4 up to 6 d Figure 3C. This result further corroborates that N.

All proteins were detected on immunoblots using two different epitope tags Figure 3E. The same complementation assay was performed expressing untagged proteins with identical results.

We concluded that tomato EDS1 family proteins can functionally replace N. Taking together the observations 1 that pad4 mutant N.

B Functionality and genetic dependency of Roq1 in Arabidopsis. Symptom development was documented 3 dpi. At least eight independent T 1 plants were tested for each genotype per replicate, and the experiment was conducted three times with similar results.

In a complementary approach, the Roq1 gene was transferred into Arabidopsis wild-type and eds1 , sag , and pad4 mutant lines.

Roq1 was expressed under control of the Arabidopsis RPS6 promoter and the octopine synthase terminator ocs, Agrobacterium tumefaciens.

T 1 transgenic seeds from transformation of the Roq1 expression constructs into Col-0 wild type and eds , pad and sag mutant lines were selected by FAST seed coat fluorescence Shimada et al.

If Roq1 can function in Arabidopsis, we expected to generate resistance to Pst DC, which is an aggressive pathogen in accession Col Indeed, severe tissue collapse was observed in Col-0 plants infected with DC at 3 d post inoculation dpi , whereas plants containing the Roq1 transgene were mostly asymptomatic Figure 4B.

In agreement, tissue collapse similar to that of Col-0 plants was observed in eds pRPS6:Roq1 plants.

The pad transgenics containing the pRPS6:Roq1 transgene behaved similar to eds transgenics and Col-0, whereas transgenic lines in the sag background were as resistant as wild-type plants expressing Roq1 Figure 4B.

Similar results were obtained when Roq1 was expressed under the control of a Ubiquitin 10 promoter fragment. Alternatively, functional recruitment to immune signaling might occur by different mechanisms in these species.

We first attempted to restore XopQ-induced cell death in eds1 or pss mutant N. Arabidopsis and tomato orthologs were expressed to similar levels Supplemental Fig.

Indicated proteins were expressed by agroinfiltration either in eds1 or pss mutant lines, and phenotypes were documented 7 dpi.

Arabidopsis proteins were expressed with or without an epitope tag, and images originate from untagged proteins Supplemental Figure 6 shows details on T-DNA constructs and protein detection.

Col eds pad double mutant was transformed with constructs for expression of EDS1 and PAD4, either from Arabidopsis or tomato and with or without an epitope tag Supplemental Figure 6C and under control of Arabidopsis promoter fragments.

True leaves were used for Trypan Blue staining 7 dpi. At least four independent T 2 populations were tested for each construct with similar results.

Lines expressing untagged proteins were used for infection assays. Lines expressing epitope-tagged proteins were used for immunodetection Supplemental Figure 6D.

Reciprocally, the Arabidopsis eds pad double mutant line was transformed with constructs encoding EDS1 and PAD4 from Arabidopsis or tomato and under control of the corresponding native promoter elements from Arabidopsis Supplemental Figure 6C.

For each transformation, several independent T 2 populations were tested for complementation of the eds pad immunity defects by infection with Hpa isolate Cala2.

In simultaneously generated transgenics expressing epitope-tagged variants, EDS1 and PAD4 from Arabidopsis and tomato accumulated to similar levels as assessed by immunodetection Supplemental Figure 6D.

Again, all proteins were detected in transgenic plants expressing epitope-tagged variants Supplemental Figure 6F.

We propose that additional factors, divergent between species as a result of coevolution with EDS1 complexes, are required by these heterocomplexes to mediate immune responses.

One rationale for genetic dissection of the EDS1 gene family in N. Amino acids targeted by mutagenesis are shown as sticks and are highlighted in pink EDS1 and orange SAGb , respectively.

Sl SAGb is shown in ribbon presentation green. EDS1 residues functionally interrogated by mutagenesis are marked. Indicated proteins were co expressed in N.

D Functionality of EDS1 variants affected in heterocomplex formation. Indicated variants as in [C] , with C-terminal 6xHA were coexpressed with XopQ-myc in eds1 mutant plants, and plant reactions were documented 7 dpi.

F Functionality of SAGb variants affected in heterocomplex formation. To validate the structural models and the N. In accordance with complex formation being progressively impaired, Sl EDS1 variants also lost their activity for restoring XopQ-induced cell death when coexpressed in eds1 mutant N.

Next, we set out to identify additional functionally relevant features of EDS1-SAGb complexes a summary of tested variants is shown in Supplemental Table 1.

We first focused on several positively charged residues lining an assumed cavity on the heterodimer surface Wagner et al. We propose that functional relevance of the positively charged residues might be masked by overexpression in the N.

F64 is a single, conserved residue exposed on the N-terminal lipase-like domain of EDS1 and framing the assumed cavity Figure 6B.

F is exposed in the monomeric EDS1 but fully buried by the association with SAG and was used to probe the importance of the C-terminal interaction surface.

D connects the N- and C-terminal domains and might mediate crosstalk at the domain interface. We also tested FD and FA variants.

Tissues were used 3 dpi for StrepII purification. B Immune activities of EDS1 variants. Plant reactions were documented 7 dpi.

In the heterodimer, an N-terminal interface is formed by the hydrolase-like domains, and a C-terminal interface is formed by the EP domains.

D Heterocomplex formation by SAG chimeric proteins. Tissues were used 3 dpi for StrepII-purification. E Functionality of SAG chimeric proteins.

Indicated proteins were expressed together with XopQ-myc in pss mutant plants by agroinfiltration. Although differences between SAGa and SAGb isoforms remain unclear, we hypothesized that if the EP domain surface is crucial for immune functions, only the chimeric protein carrying the C terminus of SAGb might be functional, whereas both chimeras should engage in heterocomplexes with EDS1.

Chimeras and native SAG isoforms were coexpressed with XopQ in pss mutant plants to test for functionality. SAGa and the Nb-Ca chimeric protein did not show any activity.

As the most probable mode of action of EDS1 complexes in immune signaling, we propose that further interaction partners might be recruited via the heterodimeric EP domain surface.

A role for NbSAG in effector-triggered immunity was also reported in previous virus-induced gene-silencing-based analyses Pombo et al.

Therefore, PAD4 might have evolved by a unique path or mechanism in Brassicaceae , supporting the hypothesis that PAD4 immune-signaling functions in this family might represent a notable exception.

It is interesting to note that NbPAD4 is induced concomitantly with Nb EDS1 under infection conditions, although it does not contribute to immune signaling in our experiments.

Competition of interactors for the EDS1 moiety for complex formation is supported by enhanced XopQ-induced cell death observed in reconstitution assays in pss mutant plants in comparison to eds1 mutant plants, but overexpression of Sl EDS1 together with Sl SAGb without XopQ was not sufficient for cell-death induction.

Future reverse genetic studies are needed to clarify which EDS1-containing heterocomplexes function predominantly in TNL signaling.

Absence of the SAGa isoform in C. This finding argues against SAGa representing merely a duplicated gene but rather supports distinct functions of individual isoforms.

We did not analyze functional relevance of EDS1 subcellular distribution in N. In interactions of Arabidopsis with P.

Similarly, an eds1 mutant tomato line sun was more susceptible to several virulent pathogens, including Xcv Hu et al. Moreover, basal resistance to several viral or other bacterial isolates was not impaired in EDS1 -silenced N.

These results do not support a general role of EDS1 complexes in basal immunity. Although incomplete N. The N. We mutagenized key residues within the interface and showed that higher order mutants containing multiple amino acid exchanges fail to function in immune signaling.

Stuttmann and J. Parker, unpublished data. Consequently, extension of the interface analysis from EDS1 to its interaction partner SAG lends important support to the previous notion that complex formation is a prerequisite for immune signaling.

Beyond the N-terminal interaction surface, five highly conserved positions distributed all along the surface of EDS1 in the vicinity of the interface with SAGb were tested for functional significance in the N.

R and K are located in a large conserved cavity formed in EDS1 heterodimeric assemblies, and homologous positions are crucial for full EDS1 immune activities in Arabidopsis Bhandari et al.

However, we did not observe reduced immune functions for corresponding Sl EDS1 variants. D was targeted to probe potential domain crosstalk within EDS1.

The F variants suggest a crucial role of the C-terminal EP domains for immune signaling Wagner et al. A most plausible hypothesis is that the large surface on Sl EDS1 delimited by F and F64 is required for interactor recruitment to mediate immune signaling.

Recruitment of protein interactors is also supported by results of our cross-species transfer of Roq1 and EDS1 family genes.

In line with previous reports showing that even immune receptors from evolutionarily distant species maintain functionality when introduced into new plant lineages Maekawa et al.

Consequently, NLR functions are generally conserved, but EDS1 complexes functionally diverged in different species, which suggests coevolution of signaling-competent heterodimeric assemblies with additional factors, most likely protein interactors.

One expectation would be that mutant lines deficient in interactors required for EDS1 immune signaling are impaired in TNL-mediated resistance responses.

Thus, proteins of the NRG1 class of helper CNLs may represent plausible candidate interaction partners, which might be recruited by EDS1 complexes to form a functional signaling module.

It should be noted that co-IPs in the report by Qi et al. Nicotiana benthamiana wild-type plants and the published eds1a-1 and pad single and eds1a-1 pad double mutant lines were used Ordon et al.

Arabidopsis Arabidopsis thaliana wild-type accession Columbia and the previously published eds pad double, eds pad sag triple mutant Feys et al.

For bacterial growth assays, the Xcv strain Thieme et al. For each time point and strain, samples were taken from at least four independent leaves and treated as biological replicates.

Bacterial growth assays were repeated at least three times with similar results. Hyaloperonospora arabidopsidis isolate Cala2 was used for infection of Arabidopsis plants, and infections were done as described previously by Stuttmann et al.

True leaves were stained with Trypan Blue 7 dpi, and representative micrographs are shown. For the verification of N.

A phylogenetic tree was calculated using a procedure derived from the SeaView graphical user interface Gouy et al. Then, the tree was computed with the PhyML program version 3.

Branch support for the final tree was computed with PhyML using 1, bootstrap replicates. The final tree was visualized with iTol Letunic and Bork, The associated multiple sequence alignment was visualized with Jalview Waterhouse et al.

For transient Agrobacterium -mediated expression of proteins in N. All constructs for expression of proteins in N.

Phenotypic assays were conducted at least 4 times, and representative results are shown. For immunodetection of proteins in support of reconstitution assays and for copurification, proteins were expressed without XopQ to avoid interference due to the negative effect of XopQ recognition on Agrobacterium -mediated protein expression Adlung and Bonas, but using the same N.

Leaf tissue was ground in liquid nitrogen, powder was resuspended in Laemmli buffer, and proteins were denatured by boiling before SDS-PAGE for immunodetection.

For StrepII purifications, 1 g of leaf tissue was ground in liquid nitrogen, and the leaf powder was resuspended in 2.

Suspensions were cleared by centrifugation and supernatants passed through a 0. Secondary antibodies were coupled to horseradish peroxidase GE Healthcare or alkaline phosphatase Sigma-Aldrich.

Protein gel blots were used for verification of protein expression in support of reconstitution assays, and protein copurification assays were repeated at least three times with similar results.

Arabidopsis plants were transformed as previously described Logemann et al. For transformation of N. Explants were surface-sterilized, and transgenic plants were regenerated.

A detailed protocol is provided as an online resource dx. Details on constructs, target sites for editing of N.

T 0 plants were tested phenotypically by challenge inoculation with XopQ-translocating P. A transgene-free pad saga-1 sagb-1 pss triple mutant was isolated from a segregating T 1 population by PCR screening and crossed to wild type for isolation of the pad sagb-1 double mutant line.

Homozygous, nontransgenic seed lots were used for experiments. For the roq1 mutant line, a T 1 population segregating for two different disruptive alleles roq and roq ; Supplemental Figure 4 was used for infection assays.

Images were taken on a LSM laser scanning microscope Zeiss. For imaging of GFP and mCherry, fluorophores were excited with nm and nm laser lines, and emission detected at nm and nm, respectively.

For localization studies, at least three independent experiments were conducted, and multiple images were taken for each replicate and each construct.

Similar results were obtained in all experiments. Nuclei were selected for measurements. Constructs were generated by Golden Gate Engler et al.

Novel level 0 modules were generated as described previously, and restriction sites were eliminated by site-directed mutagenesis or overlapping PCR products Engler et al.

Details on generated constructs and oligonucleotides used for cloning are provided in Supplemental Tables 2 and 3 , respectively.

Previously described Gantner et al. Respective constructs were transformed in yeast strain PJa by standard procedures Gietz and Schiestl, Plate-selected cotransformants were cultivated in liquid Synthetic Drop-Out media for 48 h, and dilution series were prepared and plated on selective media using a multipipette.

Extraction of proteins for immunodetection was performed as described previously by Kushnirov Data was visualized as a normalized expression heatmap using Spearman representation.

Expression values for different conditions were added manually. For gene expression analyses in N. Briefly, two leaf discs diameter 9 mm were frozen in liquid nitrogen, and tissues were disrupted using Zirkonia beads N Structural models of S.

All three sequence-template pairs could thus be confidently aligned using the hhpred algorithm Zimmermann et al.

Sequence conservation was calculated using the rate4site algorithm Pupko et al. Structural models and analysis are provided in Supplemental Data Set 3.

Additional sequence information is provided in Supplemental Data Set 1. Supplemental Figure 1. EDS1 family gene models from Arabidopsis, S.

Supports Figure 1. Supplemental Figure 2. Expression of tomato EDS1 family genes. Supplemental Figure 3.

Localization and complex formation of tomato EDS1 proteins. Supports Figure 2. Supplemental Figure 4. Generation of mutant lines by genome editing.

Supports Figure 3. Supplemental Figure 5. Supplemental Figure 6. Cross-species transfer of EDS1 -family genes.

Supports Figure 5. Supplemental Figure 7. Heterocomplex formation by EDS1 variants in a yeast two hybrid system.

Supports Figure 6. Supplemental Figure 8. Immune competence of further EDS1 variants. Supports Figure 7. Supplemental Table 1.

Supplemental Table 2. Plasmids used in this study. Supplemental Table 3. Oligonucleotides used in this study. Supplemental Table 4.

Supplemental Data Set 1. Protein sequences used for phylogenetic analyses and associated gene models. Supplemental Data Set 2.

Multiple sequence alignment underlying phylogenetic analyses. Extended data supporting Figure 1. Supplemental Data Set 3.

The following phenotypic, genotypic, and functional terms are of significance to the work described in this paper: RPS6 Gramene: AT4G EDS1 Gramene: At3g EDS1 Araport: At3g We thank Bianca Rosinsky for taking care of plant growth facilities and growing plants; Ulla Bonas for generous support, discussions, and critical reading of the article; Martin Schattat for assistance with FRET experiments; Magdalena Krzymowska for providing N and p50 expression constructs; and Jessica Andreani for assistance with phylogenetic analyses.

National Center for Biotechnology Information , U. Journal List Plant Cell v. Plant Cell. Published online Jul 2.

Author information Article notes Copyright and License information Disclaimer. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors www.

All rights reserved. This article has been cited by other articles in PMC. Open in a separate window. Figure 1. Figure 2. Figure 3.

Figure 4. Figure 5. Figure 6. Figure 7. Plant Transformation and Genome Editing Arabidopsis plants were transformed as previously described Logemann et al.

Protein Modeling Structural models of S. Supplemental Data Supplemental Figure 1.

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Carola Kretschmer Carola Kretschmer ist bekannt für

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